五氟苯基(PFP：Pentafluorophenyl，或又稱全氟苯基)配體提供多重保留機(jī)制包括：氫鍵作用、偶極-偶極作用、芳香性(π-π)作用，以及疏水(反相)作用，當(dāng)試圖分離異構(gòu)性的、芳香性的、或是鹵代的化合物特別有用。
含氟固定相為常規(guī)C18提供了互補(bǔ)的色譜分離選擇性，特別是當(dāng)需要分析堿性或平面芳香族化合物時(shí)。XSelectHSS PFP和CSH氟苯基色譜柱具有相同配體，但是不同的顆粒基體提供了不同的保留特性
XSelectTM HSS(高強(qiáng)度硅膠顆粒)系列HPLC色譜柱一直在不斷地 擴(kuò)展其選擇范圍，現(xiàn)在新增一種五氟苯基[PFP]鍵合相。該新 款XSelect HSS PFP色譜柱是目前穩(wěn)定性好且具重現(xiàn)性且可擴(kuò)展的以硅膠顆粒為基質(zhì)的PFP LC色譜柱。其HSS基質(zhì)及PFP 配體為您提供真正的PFP性能，同時(shí)不犧牲峰形，也不會有保留時(shí)間不重現(xiàn)的問題。
Objective To develop a stable LC-MS/MS method for the determination of iopamidol in human plasma and evaluate the performance of the method. Methods Deuterium isotope was used as internal standard. Plasma samples were pretreated by direct precipitation,separated by XSelectTMHSS PFP column and equally eluted by 10% acetonitrile solution containing 0.1% fomic acid and 5 mmol/L nonafluoropentanoic acid. The detection was performed by ionic scanning with positive-ion electrospray and the results were analyzed by multiple reaction monitoring( MRM) mode. According to the CLSI EP10-A and CLSI C62-A standards,the linear range,accuracy,precision,specificity,matrix effect and stability of the method were evaluated. Results The linear range of iopamidol determined by LC-MS/MS was 1 to 1 000 μg I/mL( calculated with iodine content). The deviations between the determined results and the theoretical target values were from -6.1% to 7.5% in low,medium and high concentrations of plasma samples( 3,50 and 800 μg I/m L).The repeatability was expressed as imprecision with CV less than 6.6%( 4.6% to 6.6%). The intermediate precision was expressed with CV less than 8.5%( 6.6% to 8.5%). The recoveries were from 92.5% to 95.9%. The matrix effects of the method were from 88.8% to 95.2%. The results of the method showed excellent selectivity,since hemolysis and lipemia did not affect the results. In terms of this method,plasma samples had better kept at room temperature for no more than 48 hours and repeatedly freeze-thaw from -80 ℃ for no more than 2 times,so that iopamidol could almost still be stably detected. Iopamidol was also stable in extracted samples at 4℃ in the autosampler for 72 hours. Conclusion A reliable LC-MS/MS method for determination of iopamidol in human plasma was established and evaluated.
品名 | 規(guī)格 | 編碼 |
XSelect HSS PFP Column | 100?, 3.5 μm, 1 mm X 150 mm, 1/pkg | 186005844 |
XSelect HSS PFP Column | 100?, 3.5 μm, 2.1 mm X 100 mm, 1/pkg | 186005849 |
XSelect HSS PFP Column | 100?, 5 μm, 2.1 mm X 150 mm, 1/pkg | 186005872 |
XSelect HSS PFP Column | 100?, 5 μm, 2.1 mm X 100 mm, 1/pkg | 186005871 |
XSelect HSS PFP Column | 100?, 3.5 μm, 3 mm X 30 mm, 1/pkg | 186005852 |
XSelect HSS PFP Column | 100?, 3.5 μm, 3 mm X 50 mm, 1/pkg | 186005853 |
XSelect HSS PFP Column | 100?, 3.5 μm, 3 mm X 75 mm, 1/pkg | 186005854 |
XSelect HSS PFP Column | 100?, 3.5 μm, 3 mm X 100 mm, | 186005855 |
XSelect HSS PFP Column | 100?, 3.5 μm, 3 mm X 150 mm, 1/pkg | 186005856 |
XSelect HSS PFP Column | 100?, 5 μm, 3 mm X 50 mm, 1/pkg | 186005875 |
XSelect HSS PFP Column | 100?, 3.5 μm, 4.6 mm X 30 mm, 1/pkg | 186005858 |
XSelect HSS PFP Column | 100?, 3.5 μm, 4.6 mm X 50 mm, 1/pkg | 186005859 |
XSelect HSS PFP Column | 100?, 5 μm, 3 mm X 100 mm, 1/pkg | 186005877 |
XSelect HSS PFP Column | 100?, 5 μm, 3 mm X 150 mm, 1/pkg | 186005878 |
XSelect HSS PFP Column | 100?, 3.5 μm, 4.6 mm X 250 mm, 1/pkg | 186005863 |
XSelect HSS PFP Column | 100?, 3.5 μm, 4.6 mm X 150 mm, 1/pkg | 186005862 |
XSelect HSS PFP Column | 100?, 3.5 μm, 4.6 mm X 100 mm, 1/pkg | 186005861 |
XSelect HSS PFP Column | 100?, 3.5 μm, 4.6 mm X 75 mm, 1/pkg | 186005860 |
XSelect HSS PFP Column | 100?, 5 μm, 4.6 mm X 30 mm, 1/pkg | 186005881 |
XSelect HSS PFP Column | 100?, 5 μm, 4.6 mm X 100 mm, 1/pkg | 186005884 |
XSelect HSS PFP Column | 100?, 5 μm, 4.6 mm X 150 mm, 1/pkg | 186005885 |
XSelect HSS PFP Column | 100?, 5 μm, 4.6 mm X 250 mm, 1/pkg | 186005886 |
XSelect HSS PFP Column | 100?, 5 μm, 2.1 mm X 30 mm, 1/pkg | 186005868 |
XSelect HSS PFP Column | 100?, 5 μm, 2.1 mm X 50 mm, 1/pkg | 186005869 |
XSelect HSS PFP Column | 100?, 3.5 μm, 2.1 mm X 75 mm, 1/pkg | 186005848 |
XSelect HSS PFP Column | 100?, 3.5 μm, 2.1 mm X 30 mm, 1/pkg | 186005846 |
XSelect HSS PFP Column | 100?, 3.5 μm, 2.1 mm X 50 mm, 1/pkg | 186005847 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 2.1 mm X 50 mm, 3/pkg | 186006815 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 3 mm X 50 mm, 3/pkg | 186006817 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 2.1 mm X 100 mm, 3/pkg | 186006816 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 4.6 mm X 100 mm, 3/pkg | 186006274 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 4.6 mm X 50 mm, 3/pkg | 186006273 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 3 mm X 100 mm, 3/pkg | 186006818 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 2.1 mm X 150 mm, 3/pkg | 186006803 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 3 mm X 150 mm, 3/pkg | 186006804 |
XSelect HSS PFP Method Validation Kit | 100?, 2.5 μm, 4.6 mm X 150 mm, 3/pkg | 186006805 |
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