合成寡核苷酸和DNA片段被應用于迅速發展的應用領域，包括作為主體或雜交探針用于治療性制劑。沃特世寡核苷酸分離技術(OST：Oligonucleotide SeparationTechnology)基于BEH雜化顆粒的反相色譜柱，以及Gen-Pak?離子交換柱，應對各種高分辨分析與實驗室規模分離挑戰所需，包括涉及各種DNA和RNA品種。
寡核苷酸分析柱 — 異乎尋常的高分辨率
沃特世寡核苷酸色譜柱裝有鍵合了C18的第二代雜化技術BEH顆粒。對去三苯甲基(detritylated，或稱脫保護)的合成寡核苷酸樣品的分離，基于成熟的離子對反相色譜法。沃特世提供1.7 μmUPLC顆粒或2.5 μm HPLC顆粒，裝填以各種不同色譜柱規格，從而靈活滿足各種實驗室規模分離或分析的不同需求，并能實現異乎尋常的樣品分辨率和卓越的色譜柱使用壽命。此外，沃特世的制造和質控測試程序，有助于確保批次之間與柱之間的性能的一致性，而無論應用的難度有多高。
■分離效率相當于或優于PAGE、CGE、或離子交換HPLC方法
■可從去三苯甲基(脫保護)的全長產物中分辨出失敗序列
■可放大的柱規格，滿足實驗室規模的分離需求
■超長的色譜柱使用壽命，降低單次分析或分離成本
■經MassPREP OST標準品質控測試，幫助確保性能穩定
Waters oligonucleotide analysis solutions start with our award-winning ACQUITY UPLC systems and industry-leading oligonucleotide columns, which together deliver the ultimate in high-resolution chromatographic performance. Add to this our many detection options, including tunable UV, photodiode array, and fluorescence detectors, as well as nominal mass and high-resolution mass spectrometry equipment—all optimized for UPLC?—and you have a comprehensive set of tools to meet all of your oligonucleotide analysis needs.
This application notebook includes 24 application notes, illustrating work using Waters fit-for-purpose analytical solutions for oligonucleotides, and is organized by:
UPLC-MS for oligonucleotides
UPLC-HRMS for oligonucleotides
UPLC-HDMS for oligonucleotides
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