CORTECS UPLC 1.6 μm實心核顆粒色譜柱。當與低擴散體積的UPLC系統使用時，它能夠提供最高級別的柱效。與粒徑相當的全多孔填料相比，CORTECS UPLC 1.6 μm實心核顆粒柱可以給出更尖銳、更窄的色譜峰。到現在我們已經發展出7種獨特的CORTECS色譜柱鍵合相，包括從反相到HILIC柱，以便分離性質不同的各種分析物。
與相似粒徑的全多孔顆粒相比，實心核顆粒因其形態特征的特殊性而更有可能獲得更高柱效。要實現這個理論柱效，還需要配合正確的色譜柱硬件和最佳的柱裝填技術。沃特世憑借UPLC柱生產經驗，采用超低擴散的色譜柱硬件與獨家柱裝填技術，配合全新CORTECS顆粒，樹立了UPLC色譜柱柱效新標準。
基于2010年沃特世成功推出的表面帶電雜化顆粒技術(CSH?)，為顆粒表面賦予少量正電荷，可以使用低離子強度的流動相(如甲酸)，對堿性化合物以及肽仍能獲得卓越的峰形與載量。
采用CORTECS HILIC色譜柱改善極性分析物的保留性能，親水作用色譜(HILIC)是一種對在反相色譜中保留性能較差的強極性分析物進行有效保留的模式。CORTECS HILIC色譜柱所含的未鍵合實心顆粒可以保留極性化合物。在反相色譜中，通常采用高比例水相流動相來保留極性分析物，HILIC則是使用含有高比例有機溶劑的流動相實現對極性分析物的保留。
背景：在液相色譜分析中，如果樣品運行過程 十分耗時，那么會由于對寶貴儀器的長 時間占用和處理樣品數目的限制，導致 實驗室整體工作效率降低。通過縮短樣 品分析時間來提高樣品通量是很困難的， 因為常常需要犧牲分離度或靈敏度等重 要色譜屬性。然而，采用CORTECS UPLC色譜柱就可以在保持峰容量的同時提高樣品通量。
解決方案：高效的CORTECS UPLC色譜柱采用了先進的 實心核顆粒和填充技術，能夠在高流速 下保持良好的分離性能。這讓色譜工作 者可以根據需要提高方法的流速，進而縮短運行時間并提高通量，而且無需犧 牲分離質量。圖1所示為使用 CORTECS UPLC C18 +色譜柱分析磺胺類藥物，并使用 全多孔C18色譜柱進行初始分離的結果比 較。CORTECS UPLC C18+色譜柱流速為初始 分離的兩倍，并按照流速變化對梯度作 出相應調整。
品名 | 編號 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 176003931 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 186008699 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 176003932 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 186008698 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 176003933 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 176003934 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 1/pkg | 186008695 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 1/pkg | 186008694 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 186008697 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 186008696 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 176003930 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 1/pkg | 186008691 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 1/pkg | 186008693 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 1/pkg | 186008692 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 186008700 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 30 mm, 1/pkg | 186008701 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 150 mm, 1/pkg | 186008705 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 100 mm, 1/pkg | 186008704 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 75 mm, 1/pkg | 186008703 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 50 mm, 1/pkg | 186008702 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 176003929 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 176003928 |
CORTECS T3 VanGuard Cartridge, 120?, 2.7 μm, 3.9 mm X 5 mm, 3/pkg | 186008507 |
CORTECS T3 VanGuard Pre-column, 120?, 1.6 μm, 2.1 mm X 5 mm, 3/pkg | 186008508 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 176003927 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 150 mm, 1/pkg | 186008500 |
CORTECS UPLC T3 Method Validation Kit, 120?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 186008536 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 186008710 |
CORTECS UPLC T3 Method Validation Kit, 120?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 186008537 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 186008709 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 186008708 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 186008707 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 186008706 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 3 mm X 100 mm, 1/pkg | 186008504 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 3 mm X 150 mm, 1/pkg | 186008505 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 30 mm, 1/pkg | 186008496 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 50 mm, 1/pkg | 186008497 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 176003894 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 75 mm, 1/pkg | 186008498 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 176003895 |
CORTECS UPLC T3 Column, 120?, 1.6 μm, 2.1 mm X 100 mm, 1/pkg | 186008499 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 176003147 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 176003148 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 186007165 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 100 mm, 1/pkg | 186007100 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 186007161 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 186007162 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 150 mm, 1/pkg | 186007102 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 186007163 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 186007164 |
CORTECS UPLC C18 VanGuard Pre-column, 90?, 1.6 μm, 2.1 mm X 5 mm, 3/pkg | 186007123 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 186007160 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 75 mm, 1/pkg | 186007099 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 50 mm, 1/pkg | 186007098 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 30 mm, 1/pkg | 186007097 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 1/pkg | 186007094 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 1/pkg | 186007093 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 186007156 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 186007157 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 186007158 |
CORTECS UPLC C18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 186007159 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 176003155 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 176003154 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 176003153 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 176003152 |
CORTECS UPLC C18 Column, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 176003151 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 186007180 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 176003168 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 176003169 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 75 mm, 1/pkg | 186007120 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 176003167 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 176003166 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 150 mm, 1/pkg | 186007122 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 100 mm, 1/pkg | 186007121 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 30 mm, 1/pkg | 186007118 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 50 mm, 1/pkg | 186007119 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 176003173 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 176003174 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 176003171 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 176003172 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 186007178 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 186007179 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 186007176 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 176003175 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 186007177 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 1/pkg | 186007113 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 1/pkg | 186007117 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 176003170 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 1/pkg | 186007116 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 1/pkg | 186007115 |
CORTECS UPLC C18+ Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 1/pkg | 186007114 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 186007182 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 1/pkg | 186007107 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 186007181 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 30 mm, 1/pkg | 186007108 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 50 mm, 1/pkg | 186007109 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 186007185 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 186007184 |
CORTECS UPLC C18+ Method Validation Kit, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 186007183 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 176003165 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 176003164 |
CORTECS UPLC C18+ VanGuard Pre-column, 90?, 1.6 μm, 2.1 mm X 5 mm, 3/pkg | 186007125 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 176003161 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 1/pkg | 186007104 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 176003160 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 1/pkg | 186007103 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 1/pkg | 186007106 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 176003162 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 1/pkg | 186007105 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 176003159 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 176003157 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 176003158 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 176003156 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 150 mm, 1/pkg | 186007112 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 100 mm, 1/pkg | 186007111 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 75 mm, 1/pkg | 186007110 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 1/pkg | 186008401 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 1/pkg | 186008402 |
CORTECS C8 Column, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 176003837 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 1/pkg | 186008400 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 1/pkg | 186008399 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 1/pkg | 186008398 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 176003830 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 176003831 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 176003832 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 176003833 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 186007169 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 186007166 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 50 mm, 3/pkg | 186007167 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 186007168 |
CORTECS UPLC HILIC Column, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 176003163 |
CORTECS UPLC HILIC VanGuard Pre-column, 90?, 1.6 μm, 2.1 mm X 5 mm, 3/pkg | 186007124 |
CORTECS C8 Column, 90?, 1.6 μm, 3 mm X 100 mm, 1/pkg | 186008411 |
CORTECS C8 Column, 90?, 1.6 μm, 2.1 mm X 30 mm, 3/pkg | 176003829 |
CORTECS C8 Column, 90?, 1.6 μm, 3 mm X 150 mm, 1/pkg | 186008412 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 3 mm X 100 mm, 3/pkg | 186007174 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 3 mm X 150 mm, 3/pkg | 186007175 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 186007172 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 3 mm X 75 mm, 3/pkg | 186007173 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 186007170 |
CORTECS UPLC HILIC Method Validation Kit, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 186007171 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 2.1 mm X 150 mm, 3/pkg | 176003931 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 100 mm, 3/pkg | 186008699 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 30 mm, 3/pkg | 176003932 |
CORTECS UPLC Shield RP18 Method Validation Kit, 90?, 1.6 μm, 2.1 mm X 75 mm, 3/pkg | 186008698 |
CORTECS UPLC Shield RP18 Column, 90?, 1.6 μm, 3 mm X 50 mm, 3/pkg | 176003933 |
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